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Detection of Mycoplasma pneumoniae in simulated clinical samples by Polymerase Chain Reaction
Author(s) -
JENSEN JORGEN SKOV,
SONDERGÅDANDERSEN JAN,
ULDUM SØREN A.,
LIND KLAUS
Publication year - 1989
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1989.tb00516.x
Subject(s) - mycoplasma pneumoniae , polymerase chain reaction , microbiology and biotechnology , throat , detection limit , biology , mollicutes , mycoplasmataceae , virology , mycoplasma , real time polymerase chain reaction , medicine , chemistry , gene , chromatography , pneumonia , genetics , anatomy
Polymerase chain reaction (PCR) was used to detect Mycoplasma (M) pneumoniae DNA in simulated clinical samples. Throat swabs were mixed with known amounts of broth‐grown M. pneumoniae cells. An estimated detection limit of less than 40 colony forming units (cfu) was obtained without the need for time‐consuming hybridization. The PCR is completed in one day and may be useful for the early detection of M. pnuemoniae in clinical samples.