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Two species of the phosphoprotein mRNA with differently edited reading frames discovered in measles virus infected Vero cells
Author(s) -
KALLAND KARLH.,
HÅVARSTEIN LEIV SIGVE,
ENDRESEN CURT,
HAUKENES GUNNAR
Publication year - 1989
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1989.tb00489.x
Subject(s) - phosphoprotein , measles virus , nucleic acid sequence , messenger rna , open reading frame , biology , amino acid , peptide sequence , virology , nucleotide , microbiology and biotechnology , complementary dna , virus , reading frame , morbillivirus , biochemistry , gene , measles , vaccination
One cDNA clone representing the phosphoprotein mRNA sequence of the Edmonston strain of measles virus contained 4 G nucleotides at a particular position. Two other clones contained 3 G nucleotides at the same position. Otherwise the nucleotide sequences were identical. The mRNA with 3 G nucleotides codes for the 70 kD phosphoprotein. The mRNA with 4 G nucleotides may code for a putative new peptide with 231 aminoterminal amino acids in common with the P protein whereas the 68 carboxyterminal amino acids are different from any amino acid sequence of the phosphoprotein. Thus the consequence of the insertion of one additional G is a translational shift to a shorter open reading frame. There are several indications that the observation is of biological significance.