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Different effects of ethanol on particle phagocytosis via different receptors in human monocytes
Author(s) -
MØRLAND HENNING,
MØRLAND BERIT
Publication year - 1989
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1989.tb00488.x
Subject(s) - phagocytosis , receptor , ethanol , immunology , chemistry , microbiology and biotechnology , biology , biochemistry
Endocytosis of test particles by human blood monocytes (Mo) was tested in the presence of ethanol (80 mM). Phagocytosis via the Fc (IgG)‐ or C 3 b receptors (R) was assessed by an assay in which IgG‐ or C 3 b coated sheep erythrocytes (E) were used as test particles. Latex particles were tested in parallel with opsonized E. Phagocytosis of IgG‐E was reduced to 67 ± 5% of control (= without ethanol), while the corresponding value for C 3 b‐E was 164 ± 26% of controls. Phagocytosis of latex particles was not affected by ethanol exposure (91 ± 8% of control). The receptor functions were also tested without ethanol present during the assays. In this part of the study, Mo were incubated with or without ethanol in autologous serum for 15 min at 37 °C. After washing the cells free of ethanol, binding properties of the Fc‐R or C 3 b‐R were assessed by a rosette assay. Preincubation with ethanol reduced Fc‐R binding, while attachment to C 3 b‐R seemed to be more effective. The experiments thus indicate different effects of ethanol treatment in vitro on phagocytic receptors in human Mo. Control experiments revealed no direct effect of ethanol on the test particles.