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Determination of susceptibility of anaerobic bacteria to beta‐lactam antibiotics by a tablet diffusion test
Author(s) -
JANSEN JENS E.,
BREMMELGAARD ANNIE
Publication year - 1988
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1988.tb05330.x
Subject(s) - anaerobic bacteria , cefoxitin , microbiology and biotechnology , carbenicillin , cefuroxime , piperacillin , cefotaxime , penicillin , antibiotics , ampicillin , clindamycin , agar diffusion test , chemistry , agar dilution , bacteria , biology , minimum inhibitory concentration , antibacterial activity , staphylococcus aureus , genetics , pseudomonas aeruginosa
A standardized tablet diffusion test and a reference agar dilution test was evaluated for susceptibility testing of anaerobic bacteria to beta‐lactam antibiotics. 74 freshly isolated anaerobic bacteria and three control strains ( Cl. perfringens ATCC 13124 B. fragilis ATCC 25285, B. thetaiotaomicron ATCC 29741) were tested. The in vitro activities of 7 beta‐lactam antibiotics were compared with metronidazole and clindamycin. Most active were metronidazole and clindamycin. Cefoxitin had the best activity of the beta‐lactam antibiotics, whereas piperacillin and carbenicillin had good activities. High resistance rates were found for penicillin, ampicillin, cefuroxime and cefotaxime. MIC on control strains fell well within range set by the National Commitee for Clinical Laboratory Standards (NCCLS). Correlation between MIC and inhibition zone diameters was generally good. Tablet diffusion can be used to divide anaerobic bacteria into three susceptibility categories. In addition all bacterial strains were tested for production of beta‐lacmase by a nitrocefin tube test. Beta‐lactamase production by the nitrocefin test indicated reduced sensitivity to beta‐lactam antibiotics.