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Immune hemolytic assay for identification of human anti‐dsDNA antibodies with DNA‐coated red blood cells as target cells
Author(s) -
LOFTAGER METTEK.,
ANDERSEN V.,
KOCH C.,
HELLUNGLARSEN P.
Publication year - 1988
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1988.tb05290.x
Subject(s) - immune system , antibody , dna , immunology , identification (biology) , biology , red blood cell , microbiology and biotechnology , virology , genetics , botany
A hemolytic assay was developed with the primary aim of being able to identify human lymphocytes producing anti‐dsDNA antibodies found in patients with systemic lupus erythematosus (SLE). The coating of sheep red‐blood cells with DNA was performed after precoating the cells with poly‐L‐lysine. The DNA‐SRBC were lysed by anti‐DNA antibodies from SLE sera, and the percent hemolysis was found to correlate with the anti‐DNA activity demonstrated by the Farr assay (r=0.87). Single‐stranded DNA at the surface of the coated cells could be removed after digestion with nuclease S 1 . The effect of the digestion was verified by SLE serum specific for single‐stranded DNA. With slight modifications, the target cells may be used to determine not only the titer of anti‐DNA antibodies but also the complement‐consumption and immunoglobulin classes of the anti‐DNA antibodies.