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Binding of E. Coli to human blood leukocytes
Author(s) -
YuanZhi Guan,
Kharazmi Arsalan
Publication year - 1988
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1988.tb00981.x
Subject(s) - antiserum , escherichia coli , microbiology and biotechnology , homologous chromosome , biology , strain (injury) , binding site , bacteria , enterobacteriaceae , antigen , immunology , biochemistry , genetics , gene , anatomy
The spontaneous binding of both piliated and non‐piliated E. coli to human lymphocytes, monocytes, and polymorphonuclear leukocytes (PMN) was investigated. It was found that the piliated E. coli showed a higher binding percentage to lymphocytes than the nonpiliated bacteria. A specific antiserum raised in rabbit against E. coli strongly inhibited the binding of the homologous E. coli strain to lymphocytes and monocytes. On the other hand there was no difference in the binding of piliated and non‐piliated E. coli to PMNs, and the specific E. coli antiserum did not inhibit the binding. The binding of E. coli to human lymphocytes obtained from healthy donors was investigated in a 3‐week follow‐up experiment. It was shown that there were significant differences between the binding of cells from different individuals, whereas there were no differences in the binding ability of the cells from the same individual in the 3‐week follow up.