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Enzyme‐linked immunosorbent assay compared with indirect immunofluorescence test for detection of Pneumocystis carinii specific immunoglobulins G, M, and A
Author(s) -
Nielsen Peder Bo,
Mojon Madeleine
Publication year - 1988
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1988.tb00924.x
Subject(s) - pneumocystis carinii , immunofluorescence , antigen , antibody , direct fluorescent antibody , microbiology and biotechnology , biology , virology , immunology , human immunodeficiency virus (hiv) , pneumocystis jirovecii
An ELISA to measure Pneumocystis carinii ‐specific IgG, IgM, and IgA has been developed. The antigen was prepared from purified cysts by sonication and ultracentrifuation. Antigen particles with sedimentation coefficients less than 165 S were used. The technique has been compared with indircect immunofluorescence, using whole cysts as antigen. Ninety human sera were assayed. The results were significantly correlated. The ELISA‐technique was more sensitive, and owing to the soluble antigen the daily variation was less than 1 per cent. The technique is useful for quick and reliable detection of Pneumocystis carinii antibodies in a routine laboratory.