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Lipoxygenase products in experimental septic arthritis in dogs
Author(s) -
Herlin T.,
Fogh K.,
Knudsen V. E.,
Hansen E. S.,
Bunger E.,
Ewald H.,
Holm I.,
Kragballe K.
Publication year - 1988
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1988.tb00916.x
Subject(s) - septic arthritis , high performance liquid chromatography , metabolite , chemistry , chromatography , lipoxygenase , arachidonic acid , arthritis , synovial fluid , staphylococcus aureus , leukotriene b4 , inflammation , medicine , enzyme , bacteria , biochemistry , osteoarthritis , biology , pathology , alternative medicine , genetics
Induction of an experimental septic arthritis was performed in the juvenile dog knee by an intra‐articular injection with Staphylococcus aureus. From plasma, lipoxygenase (LO) products of arachidonic acid were separated by reversed‐phase high‐performance liquid chromatography (RP‐HPLC) after extraction of lipids. The plasma samples did not contain UV‐detectable amounts of LO products other than the LTB 4 metabolite 20‐COOH‐LTB 4 , and 12‐HETE. Small amounts of chemokinetically active material were found coeluting with the eluate fraction of 20‐COOH‐LTB 4 , LTB 4 and 12‐HETE after RP‐HPLC. After 48 hours of septic arthritis, resulting in a massive acute joint inflammation, no significant changes in LO proucts were observed.