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TOXIC EFFECTS OF LIPOPOLYSACCHARIDE FROM BACTEROIDES INTERMEDIUS AND ESCHERICHIA COLI ASSESSED IN THE PRE‐IMPLANTATION MOUSE EMBRYO CULTURE SYSTEM
Author(s) -
Storeng RITSA,
Johne BERIT
Publication year - 1987
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1987.tb03101.x
Subject(s) - microbiology and biotechnology , escherichia coli , embryo , lipopolysaccharide , bacteroides , biology , escherichia , bacteria , immunology , genetics , gene
A comparative study on the toxic effects of endotoxin (lipopolysaccharide, LPS) from the strictly anaerobic Bacteroides intermedius BM1 and from Escherichia coli 055 was performed. Pre‐implantation mouse embryos at the 2‐cell stage from LPS responder (C57BL/6J) and low responder (C3H/Hej) mouse strrains were exposed to the endotoxins, and development was observed during 72 h in vitro. Toxic effects of both endotoxins on embryos from both mouse strains were demonstrated. B. intermedius LPS was less toxic than E. coli LPS. The C3H/Hej embryos were more susceptible to endotoxins than the C57BL/6J embryos. A biphasic dose‐response relationship was observed, as 100 μg/ml LPS was less embryotoxic than 1, 10 or 500 μg/ml, suggesting more complex mechanisms of effect than non‐specific cell toxicity.