Premium
ANTIBODY RESPONSE TO WHOLE CELLS AND TEICHOIC ACID OF STAPHYLOCOCCUS AUREUS STRAIN E 1369 IN HUMAN SERA
Author(s) -
JarlØV J. O.,
Espersen F.,
Christensson B.,
Jensen B. A.,
HedstrÖM S. Å.,
Hertz J. B.
Publication year - 1987
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1987.tb03097.x
Subject(s) - teichoic acid , staphylococcus aureus , microbiology and biotechnology , strain (injury) , antibody , antibody response , staphylococcus , bacteria , biology , immunology , genetics , anatomy
A whole cell Staphylococcus aureus enzyme‐linked immunosorbent assay (ELISA) using the protein A‐deficient strain E 1369 as antigen was compared to an ELISA with cell‐wall teichoic acid, extracted from the same strain, as antigen for the detection of IgG antibodies against S. aureus in human sera. A total of 178 serum samples from 119 patients with S. aureus endocarditis, complicated and uncomplicated septicaemia, non‐ S. aureus endocarditis and septicaemia were investigated together with sera from 155 healthy controls. The sensitivity in diagnosing S. aureus endocarditis was better for the whole cell‐ELISA (83%) as compared to the teichoic acid‐ELISA (61%), and the same was true for complicated septicaemia (41% vs 29%). The whole cell ELISA was also more specific for S. aureus infections with only 3.6% false positive test values compared to 7.1% for the teichoic acid‐ELISA. The differences were however, not statistically significant. None of the assays were able to distinguish between complicated and uncomplicated S. aureus septicaemia.