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FAILURE TO DETECT HYDROGEN‐SULPHIDE PRODUCTION IN LACTOSE/SUCROSE‐FERMENTING ENTEROBACTERIACEAE , USING TRIPLE SUGAR IRON AGAR
Author(s) -
Kolmos Hans Jorn,
Schmidt Jytte
Publication year - 1987
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1987.tb03092.x
Subject(s) - lactose , citrobacter freundii , fermentation , citrobacter , sucrose , enterobacteriaceae , sugar , food science , agar , salmonella , chemistry , microbiology and biotechnology , escherichia coli , biochemistry , biology , bacteria , genetics , gene
Triple Sugar Iron agar failed to detect hydrogen sulphide in 44 out of 69 hydrogen‐sulphide producing strains of Enterobacteriaceae , which at the same time fermented lactose and/or sucrose. The species involved were Salmonella typhi, Salmonella enteritidis, Citrobacter freundii, Escherichia coli , and Proteus vulgaris. By contrast, no false‐negative reactions were observed in 74 strains, which fermented neither lactose nor sucrose. Failure to detect hydrogen sulphide was probably due to acidification of the medium following the fermentation of carbohydrates. A medium without carbohydrates is preferable in diagnostic situations where hydrogen‐sulphide detection is of great importance.