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TOXINS, PUTATIVE CELL ADHESINS AND FIBRONECTIN BINDING PROPERTIES OF SALMONELLA DUBLIN
Author(s) -
Kristiansen KARINA,
Baloda SURAJ B.,
Larsen JENS L.,
WadstrÖM TORKEL
Publication year - 1987
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1987.tb03087.x
Subject(s) - bacterial adhesin , salmonella , microbiology and biotechnology , fibronectin , chemistry , cell , biology , biochemistry , bacteria , genetics , escherichia coli , gene
Fifty Salmonella dublin strains isolated from cattle and human diarrhoeal cases were assayed for toxin production, haemagglutination, cell‐surface hydrophobicity and fibronectin‐binding properties. Most strains (65% of tested) produced cytotonic toxins and cytotoxic factors when tested on Chinese hamster ovary (CHO) cells and rabbit skin test. However, only three strains produced a skin‐permeability factor as determined in pig skin intra‐dermal tests. None of the strains were positive in pig intestinal loop tests. Six of the 32 strains tested for 125 I‐fibronectin and its 125 I‐29 kDa N‐terminal domain binding showed 10–17% and 6–10% binding, respectively. Most of the strains expressed mannose‐sensitive haemagglutination (MSHA) (76%) and high cell‐surface hydrophobicity (74%) when grown at 37 °C. At 20 °C the expression of MSHA and especially the expression of high cell‐surface hydrophobicity were reduced. Twelve strains grown at 37 °C did not haemagglutinate erythrocytes from five animal species used in this study, while six of these strains expressed high cell‐surface hydrophobicity. Salmonella dublin strains isolated in Denmark appeared to express a higher frequency of fimbriae type 1 (MSHA) and a lower frequency of high cell‐surface hydrophobicity than the strains from external sources.