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EFFECT OF LEUKOCYTE AND OTHER TISSUE DIALYSATES ON NBT REDUCTION AND PROSTAGLANDIN PRODUCTION IN MICE
Author(s) -
KarhumäKi Eliisa
Publication year - 1987
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1987.tb00038.x
Subject(s) - spleen , chemistry , macrophage , superoxide , prostaglandin e2 , prostaglandin , sephadex , monocyte , prostaglandin e , biochemistry , endocrinology , immunology , biology , enzyme , in vitro
The effects of human leukocyte, porcine spleen and bovine liver dialysate fractions on Listeria resistance were measured by survival studies and by assessing the capacity of peritoneal macrophages to produce superoxide anion (O 2 ‐) and prostaglandins. Leukocyte (DLE) and other tissue dialysates were fractionated on a Sephadex G‐10 column. Thereafter the significant activities were found in fraction III of DLE, fraction II of porcine spleen and fraction II + III of bovine liver dialysate. The treatment with active porcine spleen dialysate fraction increased the capacity of peritoneal macrophages to generate superoxide anion. On the other hand, this fraction significantly decreased the production of prostaglandin PGE 2 and thromboxan B 2 . These results may indicate that all dialysates can be a source of a non‐specifically‐acting immunomodulatory preparation and that the infection‐resistance‐increasing substances seem to operate via the monocyte/macrophage activation.