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RECOMBINANT INTERLEUKIN 2 INDUCES PROLIFERATION AND DIFFERENTIATION OF HUMAN B LYMPHOCYTES
Author(s) -
Punn Juha,
Eskola Jussi
Publication year - 1987
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1987.tb00026.x
Subject(s) - pokeweed mitogen , microbiology and biotechnology , biology , recombinant dna , cell growth , secretion , population , stimulation , antibody , t cell , in vitro , immunology , immune system , endocrinology , medicine , peripheral blood mononuclear cell , biochemistry , environmental health , gene
Highly purified normal human tonsillar B lymphocytes were cultured with recombinant IL 2 (rIL 2). The responses were minimal when the cells were cultured with rIL 2 alone. Dose‐dependent proliferation and differentiation into immunoglobulin‐secreting cells were found when the cells were simultaneously activated with Staphylococcus aureus Cowan I (SAC). T cells were not found to proliferate in these cultures. B‐cell responses were not augmented when 16% T cells were added to the B‐cell population. In contrast, B‐cell proliferation and Ig secretion were significantly enhanced by adding 0,5% T cells when cultured in the presence of pokeweed mitogen (PWM). Moreover, B‐cell stimulation by rIL 2 was detected at very low cell densities. It is concluded that IL 2 directly affects the function of activated B cells.