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RHEUMATOID FACTORS IN PSORIATIC SCALE, SERUM AND CIRCULATING IMMUNE COMPLEXES DETECTED BY AN ISOTYPE‐SPECIFIC ELISA
Author(s) -
åSbakk Kjetil,
Iversen OleJan
Publication year - 1987
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1987.tb00025.x
Subject(s) - isotype , rheumatoid factor , titer , immunology , antibody , agglutination (biology) , medicine , immune system , direct agglutination test , microbiology and biotechnology , monoclonal antibody , biology , serology
An isotype‐specific ELISA for detection of rheumatoid factors (RFs) is described. The assay utilizes rabbit anti‐albumin IgG bound to albumin as solid phase reactant (test wells). RF‐activity was expressed as the difference in reaction between test wells and wells containing F(ab′) 2 ‐fragments of the rabbit IgG bound to albumin. The activity correlated well with the conventional Waaler agglutination test on sera that showed a positive Waaler titre, but several sera that were negative by the Waaler test were positive for RF of IgG isotype by the ELISA‐test. The method was applied on specimens from psoriatic patients, and RFs of IgG‐and IgA isotype were demonstrated in serum immune complexes from all the psoriatic patients examined. IgG‐ and IgA‐RF was also demonstrated in extracts from psoriatic scale, and RFs in scale were demonstrated independently of free rheumatoid factor activity in serum.