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ASIALO GM1 + NK CELLS HAVE OPPOSITE ROLES IN THE ACTIVATION OF ALLOREACTIVE CYTOTOXIC T LYMPHOCYTE (CTL) RESPONSE IN VITRO AND IN VIVO
Author(s) -
Varkila K.,
Silvennoinen O.,
Hurme M.
Publication year - 1987
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1987.tb00022.x
Subject(s) - cytotoxic t cell , in vivo , in vitro , ctl* , microbiology and biotechnology , biology , immunology , biochemistry
The immuno‐regulatory role of AsialoGM1 + murine NK cells in the induction of allogeneic cytotoxic T cell responses was studied in vivo and in vitro. Depletion of ASGM1 + cells from the (CBAxC57BL/6)F 1 cells used for the foot‐pad immunization of CBA mice greatly reduced the formation of allospecific CTLs. However, highly purified ASGM1 + cells were not efficient stimulators of alloCTLs in vivo by themselves. When the same genetic combination was used in alloCTL stimulation culture in vitro , ASGM1 + cells were seen to suppress the activation of the CTL response. The opposite roles of ASGM1 + cells in the alloCTL activation in vivo and in vitro were seen when both lymphoid cells (spleen cells or peripheral blood cells) and non‐lymphoid cells (epidermal cells) were used as stimulators. The data presented in this paper suggest that during the alloCTL activation, ASGM1 + cells have an important enhancing role in vivo ; but in vitro these cells suppress the antigen‐presenting cells.