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SAME‐DAY CONFIRMATION OF STAPHYLOCOCCUS AUREUS BACTERAEMIA BY A THERMONUCLEASE TEST
Author(s) -
Bergh Kåre,
MæLand Johan A.
Publication year - 1986
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1986.tb03055.x
Subject(s) - staphylococcus aureus , microbiology and biotechnology , coagulase , biology , agar plate , incubation , agar , bacteria , staphylococcus , medicine , genetics , biochemistry
A test to detect thermostable DNase activity (TDNase test) was evaluated for rapid confirmation of S. aureus bacteraemia, using toluidine blue O DNA agar plates for the testing. A total of 226 blood cultures which grew bacteria were examined. S. aureus was identified in 76 of the cultures. All of the S. aureus isolates showed positive TDNase test after 2 h of incubation of the test plates. The remaining isolates examined, most of them coagulase‐negative staphylococci, showed a negative test. The TDNase test enables reliable same‐day confirmation of S. aureus bacteraemia.