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EFFECTS OF CARBON DIOXIDE UPON THE IN VITRO ACTIVITY OF ERYTHROMYCIN
Author(s) -
Dibb WILLIAM LAND,
Digranes ASBJØRN,
Bottolfsen KIRSTEN LILAND
Publication year - 1986
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1986.tb03038.x
Subject(s) - carbon dioxide , erythromycin , in vitro , chemistry , environmental chemistry , biochemistry , antibiotics , organic chemistry
The in vitro activity of erythromycin against clinical isolates of Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Streptococcus pyogenes and Haemophilus influenzae was examined by agar dilution and agar diffusion methods. The plates were incubated in air alone or in 8% CO 2 and air. The minimal inhibitory concentrations (MICs) measured in air alone were lower for most of the isolates, compared to those found in 8% CO 2 . The greatest differences in MIC values were found for H. influenzae ; the MIC 50% was 0.5 mg/l in air and 4 mg/l in 8% CO 2 . Sensitivity testing by the agar diffusion method (ICS) showed considerable differences between results obtained in air and in 8% CO 2 ; the inhibition zones were generally smaller in CO 2 . The most marked reduction in zone sizes after incubation in 8% CO 2 was seen with the H. influenzae isolates; 15 out of 43 isolates moved from the “sensitive” to “moderately sensitive” group. Sensitivity determination of aerobic bacteria for erythromycin should be performed in air alone in the routine laboratory.