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THE MICROSCOPIC DIAGNOSIS OF PNEUMOCYSTIS CARINII
Author(s) -
Nielsen PEDER BO,
Goyot PIERRE,
Mojon MADELEINE
Publication year - 1986
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1986.tb03015.x
Subject(s) - pneumocystis carinii , staining , grocott's methenamine silver stain , pathology , silver stain , methylene blue , chemistry , medicine , virology , human immunodeficiency virus (hiv) , pneumocystis jirovecii , biochemistry , photocatalysis , catalysis
An assessment of the Gram, the Methylene blue, and the Ziehl‐Neelsen procedures in diagnosing Pneumocystis carinii has been performed. By using heat and methanol as fixatives and phase‐contrast microscopy we found the procedures valuable in detecting the pneumocysts, especially in the Gram staining. The diagnosis should, however, be confirmed by a re‐staining with a specific staining, viz. the toluidine blue 0 or the silver impregnation a.m. Gomori‐Grocott. It is additionally shown that the staining a.m. Gomori‐Grocott is dependent on the density of the preparation.