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EFFECT OF ELEVATED TEMPERATURE ON HUMAN IMMUNOGLOBULIN SYNTHESIS, LYMPHOKINE PRODUCTION AND LYMPHOCYTE PROLIFERATION IN VITRO
Author(s) -
NÄRvÄNen OUTI,
Jokinen ILMARI,
Poikonen KARI,
RÄSÄNen LIISA,
Arvilommi HEIKKI
Publication year - 1986
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1986.tb02118.x
Subject(s) - pokeweed mitogen , phytohaemagglutinin , concanavalin a , lymphokine , in vitro , lymphocyte , stimulation , antibody , immunology , interleukin 2 , biology , chemistry , endocrinology , biochemistry
Fever in infections is mediated by endogenous pyrogen, a protein inseparable from interleukin 1 (IL‐1). It remains unclear what the role of fever in host defence is. We studied the effect of elevated temperature on mitogen‐induced human lymphocyte stimulation in vitro. Lymphocyte proliferation, elaboration of leukocyte migration inhibiting factor (LIF) and immunoglobulin production were measured at normal and elevated temperatures. Pokeweed mitogen‐(PWM) driven responses were quantitated with a plaque‐forming cell assay and by measuring secreted immunoglobulins in culture medium. The responses at 38, 39 or 40°C were, if anything, lower than those at 37°C. The production of LIF after stimulation with PWM was not augmented at elevated temperatures. In contrast, the proliferative responses to PWM, phytohaemagglutinin (PHA) and convanavalin A (ConA) were increased at 38°C. These results indicate that elevated temperature has diverse effects on mitogen‐induced lymphocyte functions in vitro.