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TOPOGRAPHICAL DISTRIBUTION OF INFLAMMATORY LEUKOCYTE SUBSETS IN ACUTE CELLULAR REJECTION OF A KIDNEY ALLOGRAFT
Author(s) -
Leskinen RITVA,
HÄYry PEKKA
Publication year - 1986
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1986.tb02092.x
Subject(s) - peripheral blood mononuclear cell , infiltration (hvac) , immunology , monocyte , cytotoxic t cell , inflammation , cellular infiltration , immunoperoxidase , biology , mononuclear cell infiltration , pathology , antibody , medicine , monoclonal antibody , in vitro , biochemistry , physics , thermodynamics
The topographical distribution of different mononuclear inflammatory cell subsets in acute cellular rejection of human renal allograft was analyzed. Marker antibodies to T cells (OKT11), T helper cells (T4), T suppressor/killer cells (OKT8), B cells (B‐1), monocytes (OKM1) and mononuclear phagocytes at different stages (FMC17, FMC33) and the indirect immunoperoxidase method were employed. At early stages of rejection, T lymphocytes were mostly confined to the perivascular areas and there was a T helper (Th) cell predominance over T suppressor/killer (Tsk) cells in the inflammatory infiltrate. Very few T cells and monocytes were found to infiltrate the arterial wall. Mononuclear phagocytes dominated over T lymphoid cells in the intertubular areas. Later during rejection, the Th/Tsk ratio became inverted, inflammatory T cells invaded also the intertubular areas, and infiltration of the arterial wall by T cells and mononuclear phagocytes became evident. Granulocytes were associated with the late events in the graft, and their appearance coincided with beginning necrosis. These non‐random features in the distribution of the inflammatory cells were most pronounced during early stages of acute cellular rejection;