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IN VITRO LOCOMOTION OF BLOOD MONOCYTES IN MILLIPORE FILTERS — EVALUATION OF THE LEADING FRONT METHOD
Author(s) -
Aasen TOR B.,
Schreiner AKSEL
Publication year - 1986
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1986.tb02088.x
Subject(s) - monocyte , coefficient of variation , in vitro , immunology , chemistry , biology , chromatography , biochemistry
Monocyte migration in millipore filters was measured by the leading front method. The precision, sensitivity and specificity of the method was assessed. The migrational response of monocytes to N‐for‐mylmethionylleucyl‐phenylalanine (FMLP) was equal in concentrations ranging from 5times10 −9 to 5times10 −7 M. No dose‐response relationship was demonstrated. The random analytical variation of the method was considerable, the mean coefficient of variation of duplicate experiments being 9.05%. The inter‐subject variation was also large. A sample size of 30 to 40 individuals would be necessary to demonstrate a 1 SD difference between individuals at a significance level of 0.05 and power of 0.80. It is concluded that the method is able to detect agents which stimulate monocytes at least equally well as FMLP, but may be impracticable for demonstrating changes in monocyte migration, or differences in the migratory response among different populations of monocytes.