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CEREBELLAR FOLIATION IN RATS
Author(s) -
CONRADI NILS G.,
MÜNTZING KRISTINA
Publication year - 1985
Publication title -
acta pathologica microbiologica scandinavica series a :pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0164
DOI - 10.1111/j.1699-0463.1985.tb03967.x
Subject(s) - sagittal plane , cerebellum , anatomy , gestation , lobe , biology , pregnancy , endocrinology , genetics
The effects of maternal malnutrition on the formation of cerebellar fissures was studied in foetal rats. The rats were given a diet containing either 14 (normal) or 7 (protein‐deprived) per cent protein by weight from two weeks before conception and throughout gestation. The first day with a sperm‐positive vaginal smear was designated as Embryonic day 1 (E 1). Foetuses were examined on days E 19 to 22. In seventy‐five foetuses, a sagittal slice of the cerebellum, 3–4 mm thick was embedded in Sorvall embedding medium. Sagittal sections, 2 μm thick were cut at levels 100 μm apart through the specimen. Sixty‐three foetuses were examined for presence of cerebellar fissures only after filling their vascular system with ink‐gelatin and cutting serial sagittal frozen sections, 100 μm thick. A morphological comparison of the foliation in normal and malnourished foetuses suggested a slight delay in the malnourished ones, albeit the earliest fissures were visible on the same day as in normal foetuses. The existence of a developmental alteration due to malnutrition was demonstrated by a decrease in the length of the pial surface and a surface folding index day on E 22. The alteration is discussed in relation to previously reported changes in cerebellar development during malnutrition, such as delayed Purkinje cell formation and alterations in proliferation/differentiation in the EGL.