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DEVELOPMENT OF EARLY POST‐ISCHEMIC INJURY IN THE LIVER AS EVALUATED BY A DOUBLE STAINING METHOD COMBINING AN INTRAVITAL DYE EXCLUSION TEST AND ALIZARIN RED S
Author(s) -
JENNISCHE EVA
Publication year - 1985
Publication title -
acta pathologica microbiologica scandinavica series a :pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0164
DOI - 10.1111/j.1699-0463.1985.tb03963.x
Subject(s) - staining , alizarin red , evans blue , perfusion , ischemia , stain , ruthenium red , pathology , vital stain , calcium , medicine , chemistry , andrology
The early development of post‐ischemic cell injury was investigated in rat livers. Rats were subjected to 90 minutes of liver ischemia, followed by periods of re‐perfusion from 10 minutes to 3 hours. The ischemia‐induced injury was quantified by using a double staining method. An intravital dye exclusion test with Evans blue was combined with a histochemical stain for calcium, Alizarin red S (ARS). It was found that the markers identified two populations of injured cells, positive for Evans blue (EBA) and ARS respectively. The number of injured cells increased successively during the re‐perfusion period. The overlapping between the two populations was small during the early post‐ischemic phase but increased with increasing re‐perfusion time. Treatment with ruthenium red, a blocker of mitochondrial calcium uptake, during the re‐perfusion period significantly reduced the number of ARS‐positive cells, while the number of EBA‐positive cells was not affected. It is suggested that the two markers used identify cell populations, which are injured by different mechanisms operating in the post‐ischemic phase. These mechanisms may or may not be dependent on calcium.