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NK‐CELL STIMULATING PROPERTIES OF A MEMBRANE PROTEOGLYCANE FROM NON‐CAPSULATED KLEBSIELLA PNEUMONIAE BIOTYPE a
Author(s) -
NORMIER GERARD,
PINEL ANNEMARIE,
D'HINTERLAND LUCIEN DUSSOURD,
RAMSTEDT URBAN,
WIGZELL HANS
Publication year - 1985
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1985.tb02951.x
Subject(s) - klebsiella pneumoniae , microbiology and biotechnology , biology , klebsiella , chemistry , escherichia coli , gene , genetics
Upon testing the individual fractions of a composite bacterial vaccine for biological activities, a potent immuno‐stimulatory capacity could be demonstrated within a crude membrane proteoglycan preparation from Klebsiella pneumoniae. One of its characteristic features was the capacity to induce alpha‐type interferon and increased NK activity in vivo in mice, following intraperitoneal or oral administration. A highly purified fraction from the crude preparation was obtained using alkaline hydrolysis, delipidation and size fractionation. This fractin was shown to be a very potent inducer of NK cells in vivo or in vitro , where in the latter systems concentrations as low as 0.1 microgramme per ml were highly efficient.