Premium
DETECTION OF ANTIBODIES TO YERSINIA ENTEROCOLITICA BY SINGLE RADIAL DIFFUSION‐IN‐GEL AND PEROXIDASE LABELLED ANTIBODIES
Author(s) -
Melby Kjetil
Publication year - 1985
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1985.tb02949.x
Subject(s) - yersinia enterocolitica , antibody , titer , agglutination (biology) , immunoglobulin m , yersinia , antibody titer , antigen , conjugate , microbiology and biotechnology , immunoglobulin g , chemistry , immunology , biology , bacteria , genetics , mathematical analysis , mathematics
Sixty‐nine sera with agglutinating antibodies to Y.enterocolitica were tested with a DIG‐ELISA method using ethanol inactivated preparations of Y.enterocolitica (0:3 and 0:9). All sera with agglutination titer ≥ 1280 were positive of which 71% were positive for IgM and IgA. Sera having lower titers by the agglutination technique were usually positive only for IgG antibodies. By titer 160 70% were positive using a conjugate directed against IgG, IgA and IgM. Further investigations are necessary to determine the clinical implication of the antibody‐response measured within these immunoglobulin classes.