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IN VITRO BLASTOGENIC RESPONSE AND IMMUNOGLOBULIN SYNTHESIS IN MURINE SPLEEN CELLS STIMULATED WITH LIPOPOLYSACCHARIDES FROM CAMPYLOBACTER JEJUNI AND CAMPYLOBACTER COLI
Author(s) -
NÆSS VIGFRID
Publication year - 1985
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1985.tb02947.x
Subject(s) - campylobacter jejuni , polyclonal antibodies , antibody , spleen , incubation , campylobacter , microbiology and biotechnology , in vitro , stimulation , biology , salmonella , immunoglobulin a , immunoglobulin m , immunoglobulin g , immunology , bacteria , biochemistry , endocrinology , genetics
Lipopolysaccharides isolated from strains of Campylobacter jejuni and Campylobacter coli were mitogenic for spleen cells as measured by 3 H‐thymidine incorporation. The incorporation was dose dependent with an increase with increased concentration of LPS. Addition of LPS beyond 500 μg/ml gave inhibition of incorporation. Stimulation of the spleen cells for 3 days with LPS led to a polyclonal activation of immunoglobulin synthesis. The amount of immunoglobulins synthesised showed a maximum between the 9th and 11th day of incubation, and another maximum between the 15th and 17th day. The immunoglobulins produced were IgM antibodies. Specific antibodies against the LPS used for the stimulation were not detected.