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THE QUANTITATION OF C3d BY ROUTINE METHODS AFTER THE DIRECT ABSORPTION OF HUMAN PLASMA WITH ANTI‐C3c
Author(s) -
MLYNEK YOUVAL,
NILSSON MARIANNE
Publication year - 1985
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1985.tb02944.x
Subject(s) - human plasma , antiserum , plasma , chromatography , absorption (acoustics) , chemistry , antibody , materials science , immunology , biology , physics , quantum mechanics , composite material
The immunological methods for quantitating C3d in plasma require first the removal of less fragmented intermediates as well as the intact C3. We describe an alternative method for the quantitation of C3d in human plasma. The components which should be removed are absorbed (precipitated) directly in the plasma by a specific anti‐C3c antiserum. It is then possible to determine the concentration of C3d by routine immunological methods.