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IMMUNOGLOBULIN SYNTHESIS IN A PATIENT WITH C4 DEFICIENCY: FUNCTIONAL IMMATURITY OF PERIPHERAL BLOOD LYMPHOCYTES
Author(s) -
SJöHOLM A.G.,
HAMMARSTRöM L.,
SMITH C. I. E.,
KJELLMAN N.I. M.
Publication year - 1985
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1985.tb02940.x
Subject(s) - pokeweed mitogen , immunology , antibody , stimulation , b cell , antigen , subclass , biology , lymphocyte , peripheral blood mononuclear cell , in vitro , endocrinology , biochemistry
Peripheral blood lymphocytes from a systemic lupus erythematosus patient with homozygous C4 deficiency were stimulated in vitro. Although T cell independent stimulation (Epstein‐Barr virus) induced normal proportions of IgM secreting cells. IgG and IgA secreting cells were almost absent. Virtually no immunoglobulin secretion was obtained after T cell dependent stimulation (pokeweeed mitogen). Cell fractionation experiments indicated that the low pokeweed mitogen response, at least with regard to IgG and IgA production, was mainly due to impaired B‐lymphocyte function. The serum IgG subclass distribution pattern of specific antibodies against certain carbohydrate antigens demonstrated a relative lack of IgG2 antibodies. The findings were consistent with functional immaturity of B lymphocytes in the patient.