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PROBLEMS IN THE DETECTION OF COMPLEMENT‐FIXING IMMUNE COMPLEXES
Author(s) -
RøDAHL EYVIND
Publication year - 1985
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1985.tb02933.x
Subject(s) - complement (music) , antibody , immune system , ankylosing spondylitis , immunology , complement system , complement c1q , chemistry , microbiology and biotechnology , medicine , biology , biochemistry , complementation , gene , phenotype
In the application of the anti‐C1q, the anti‐C3, or the conglutinin‐binding assay for the detection of complement‐fixing circulating immune complexes in patients with ankylosing spondylitis or healthy blood donors, antibodies cross‐reacting with the solid‐phase bound agent were observed in several sera, resulting in erroneous interpretation of the tests. Corrections for the interfering antibodies were made by testing for the binding to conglutinin both in the presence and in the absence of Ca**, while the anti‐C1q and the anti‐C3 assays included the application of F(ab') 2 fragments of normal IgG to check for anti‐F(ab') 2 anti‐bodies.