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EFFECT OF HUMAN LEUKOCYTE AND OTHER TISSUE DIALYSATES ON LISTERIA RESISTANCE AND PHAGOCYTOSIS IN MICE
Author(s) -
KARHUMÄKI ELIISA,
ASHORN RALPH,
KROHN KAI
Publication year - 1985
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1985.tb02931.x
Subject(s) - spleen , listeria , phagocytosis , listeria monocytogenes , sephadex , microbiology and biotechnology , kidney , splenocyte , transfer factor , fractionation , bacteria , monocyte , biology , chemistry , andrology , immunology , endocrinology , biochemistry , medicine , chromatography , genetics , enzyme
The effect of dialyzable transfer factor (TF d ) on Listeria resistance was measured by survival studies and by assessing phagocytic capacity of peritoneal macrophages. Unfractionated dialysates from human leukocytes (DLE), bovine liver, porcine spleen and kidney as well as saline were injected i.p. into NMRI mice 72 h before the i.p. injection of 1–3 times 10 6 Listeria organisms. The results show that DLE, porcine spleen and bovine liver dialysate increased the LD 50 5–20 times. Porcine kidney dialysate had no effect on the survival of the mice. After the fractionation of porcine spleen dialysate on Sephadex G–10 column, a significant activity was found in two fractions, II and IX. When active fractions were given together (II + IX) i.p. three days prior to the infection with Listeria organisms, the survival of mice increased significantly, whereas no effect was seen when the fractions were given i.v. and the bacteria i.p. Also the treatment with active fractions increased significantly the phagocytic capacity of peritoneal macrophages. Taken together, our results suggest that the Listeria protective substances seem to operate via monocyte activation.