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ANTIGENICITY OF LIPOPOLYSACCHARIDES FROM CAMPYLOBACTER JEJUNI AND CAMPYLOBACTER COLI IN PASSIVE HAEMAGGLUTINATION TESTS AND ENZYME‐LINKED IMMUNOSORBENT ASSAYS
Author(s) -
NÆSS V.,
HOFSTAD T.
Publication year - 1985
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1985.tb02929.x
Subject(s) - antigenicity , campylobacter jejuni , antiserum , heterologous , hemagglutination , microbiology and biotechnology , serology , campylobacter , biology , antibody , cross reactivity , virology , chemistry , bacteria , cross reactions , biochemistry , immunology , genetics , gene
Passive haemagglutination tests and ELISA were used to study the serological activity of homologous and heterologous rabbit antisera against LPS prepared from various strains of C. jejuni/coli. In both test systems the homologous antisera exhibited serological activity against LPS. The heterologous antisera showed some degree of intra‐and inter‐ species cross‐reactivity. The cross‐reacting was most pronounced in the ELISA. Erythrocytes sensitized with untreated LPS gave higher antibody‐titres than erythrocytes sensitized with alkali‐treated LPS in the haemagglutination tests.