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COMPLEMENT ACTIVATION BY CLUMPING FACTOR AND PROTEIN A FROM STAPHYLOCOCCUS AUREUS STRAIN E 2371
Author(s) -
ESPERSEN F.
Publication year - 1985
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1985.tb02923.x
Subject(s) - staphylococcus aureus , complement system , microbiology and biotechnology , complement factor b , alternative complement pathway , complement factor i , factor h , in vitro , strain (injury) , chemistry , blood proteins , biochemistry , biology , bacteria , immunology , antibody , genetics , anatomy
Purified surface components from Staphylococcus aureus strain E 2371, which bind to human plasma proteins, were tested for their ability in vitro to activate complement present in normal human serum. Staphylococcal clumping factor as well as protein A both isolated from strain E 2371 were capable of activate complement, while the fibronectin‐binding protein showed no activity. At identical molar concentrations clumping factor was more potent than protein A to induce complement consumption. Both clumping factor and protein A were able to activate the alternative complement pathway as demonstrated in serum chelated with ethylene‐glycerol‐tetra‐acetic acid (10 mM) in the presence of MgCl 2 (10 mM).