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The Influence of Antimicrobial Agents on Macrophage‐Associated Staphylococcus Aureus
Author(s) -
Nesthus Ingerid,
Haneberg Bjørn,
Glette Johan,
Solberg Claus Ola
Publication year - 1985
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1985.tb02875.x
Subject(s) - staphylococcus aureus , antimicrobial , microbiology and biotechnology , macrophage , anti infective agents , biology , chemistry , bacteria , in vitro , biochemistry , genetics
Macrophages obtained by culturing human blood monocytes were incubated with Staphylococcus aureus for phagocytosis to occur and exposed to gentamicin, rifampin, clindamycin or trimethoprim/sulphamethoxazole. The macrophage‐associated bacteria were protected against gentamicin at low concentrations (1 mg/l) and trimethoprim/sulphamethoxazole. However, high concentrations of gentamicin and clindamycin reduced the number of bacteria, indicating that these drugs penetrated into human macrophages and killed phagocytosed bacteria. Rifampin, even at low concentrations (0.5 mg/l), caused a marked reduction in macrophage‐associated bacteria, implying that the drug penetrated into the phagocytes and retained its effect in the cells most effectively.