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Effect of Monosaccharides and Ethyleneglycol on the Interaction Between Escherichia Coli Bacteria and Octyl‐Sepharoser̀
Author(s) -
öhman Lena,
Magnusson KarlEric,
Stendahl Olle
Publication year - 1985
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1985.tb02864.x
Subject(s) - mannose , monosaccharide , escherichia coli , fucose , bacteria , chemistry , galactose , hydrophobic effect , hydrophilic interaction chromatography , biochemistry , chromatography , biology , genetics , high performance liquid chromatography , gene
Combined effects of monosaccharides and reduced surface tension of the medium were studied in relation to the hydrophobic binding of Escherichia coli bacteria, with and without mannose‐specific structures. Hydrophobic binding was analyzed by hydrophobic interaction chromatography on Octyl‐Sepharoser̀. The results showed that ethyleneglycol, as well as mannose, reduced the hydrophobic interaction of the bacteria with mannose‐specific structures. This effect was potentiated by combining ethyleneglycol and mannose. No other monosaccharides tested (galactose and fucose) had any effect on the hydrophobic interaction of bacteria with mannose‐specific structures. These results further strengthen the hypothesis that the mannose‐specific interaction of Escherichia coli bacteria is, at least in part, mediated by hydrophobic forces.