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THE INFLUENCE OF VARIOUS FIXATIVES ON THE IMMUNOHISTOCHEMICAL DEMONSTRATION OF A NUMBER OF PLASMA PROTEINS AND ONCOFETAL PROTEINS IN PARAFFIN EMBEDDED MATERIAL
Author(s) -
JACOBSEN MARIANNE,
JACOBSEN GRETE KRAG
Publication year - 1984
Publication title -
acta pathologica microbiologica scandinavica series a :pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0164
DOI - 10.1111/j.1699-0463.1984.tb04428.x
Subject(s) - fixative , antigenicity , paraformaldehyde , chemistry , ferritin , antigen , microbiology and biotechnology , biochemistry , biology , cytoplasm , immunology , organic chemistry
The influence of different fixatives on the antigenicity of alpha‐fetoprotein (AFP), albumin, prealbumin, transferrin, ferritin, human chorionic gonadotropin (HCG), specific pregnancy beta 1 ‐glycoprotein (SP 1 ) and human placental lactogen (HPL) was studied. No single fixative was superior for all the antigens investigated. For intracellular antigens the best all‐round fixatives were, however, 10% buffered formalin, 4% buffered paraformaldehyde, 10% formol‐calcium and ethanol acetic acid (EA), cold processed. The preferable fixatives were for AFP: Bouińs fixative, B‐5 and EA; for albumin: 10% formol‐calcium; for prealbumin: Zambonis fixative and B‐5; for transferrin: EA; for ferritin: 10% buffered formalin, 4% buffered paraformaldehyde, Lillies fixative, Zambonis fixative and EA; for HCG: Bouińs fixative and B‐5; for SP 1 : Lilliés fixative and EA; and for HPL: 4% buffered paraformaldehyde and Lilliés fixative. The preservation of extracellular antigens was different from the above mentioned intracellular ones. Thus fixation in 10% buffered formalin gave poor results while the coagulant fixatives such as Bouins fixative, B‐5 and Clelands fixative in general showed good preservation of the antigenicity of the extracellular antigens.