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ANTISERUM AGAINST FORMALIN‐FIXED HUMAN MILK FAT GLOBULE GLYCOPROTEIN FOR IMMUNOHISTOCHEMISTRY OF NORMAL AND MALIGNANT APOCRINE EPITHELIUM
Author(s) -
FORSMAN LISBETH M.,
KARHI KIMMO K.,
AUTERO MATTI,
GAHMBERG CARL G.,
ANDERSSON LEIF C.
Publication year - 1984
Publication title -
acta pathologica microbiologica scandinavica series a :pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0164
DOI - 10.1111/j.1699-0463.1984.tb04412.x
Subject(s) - antiserum , apocrine , glycoprotein , immunohistochemistry , epithelium , pathology , biology , microbiology and biotechnology , mammary gland , chemistry , antibody , medicine , immunology , cancer , breast cancer , genetics
A rabbit antiserum was raised against formalin‐fixed glycoprotein isolated by lectin affinity chromatography from human milk fat globules. After adsorption witherythrocytesand normal blood leukocytes the antiserum detected in immunoblotting of fresh mammary carcinoma tissue a major component of 75 000 dalton apparent molecular weight. The antiserum specifically decorated normal and malignant apocrine epithelium in sections of formalin‐fixed tissues. The usefulness of such antisera for routine immunohistochemical diagnosis of metastatic carcinomas is demonstrated.