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THE INFLUENCE OF FORMALIN AND PARAFFIN EMBEDDING ON THE IMMUNOHISTOCHEMICAL REACTION OF MONOCLONAL ANTIBODIES APPLIED TO FEMALE BREAST TISSUE
Author(s) -
RASMUSSEN B. B.,
HILGERS J.,
HILKENS J.
Publication year - 1984
Publication title -
acta pathologica microbiologica scandinavica series a :pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0164
DOI - 10.1111/j.1699-0463.1984.tb04392.x
Subject(s) - immunoperoxidase , immunohistochemistry , monoclonal antibody , antigen retrieval , pathology , antigen , staining , antibody , frozen section procedure , biology , pronase , fixation (population genetics) , chemistry , medicine , immunology , enzyme , biochemistry , gene , trypsin
Monoclonal antibodies, raised against purified human milk fat globule membranes, were applied to human tissue and the corresponding antigenic determinants detected by an immunoperoxidase method. In human breast tissue, the presence of antigenic determinants was characterized by considerable heterogeneity although it is confined to epithelial cells and cannot be detected in non‐epithelial areas. The effect of fixation and paraffin embedding on the reaction pattern was evaluated on tissue from 25 primary breast carcinomas. For each tumor, both frozen, acetone fixed and fixed, paraffin embedded tissue was available. Four of the eight applied antibodies could detect fewer positive cells in formalinfixed tissue as compared to frozen, acetone‐fixed tissue from the same tumor. The positive peroxidase reaction was better preserved after fixation in Lillie's solution. Treatment of formalin‐fixed tissue with pronase did not give a better effect. Antibodies with preserved positive reaction in formalin‐fixed breast tissue were applied to a range of epithelial and non‐epithelial human malignant tumors of varying origin. A heterogenous positive reaction could be detected in the majority of tumors derived from epithelial cells, but not in non‐epithelial tumors.

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