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HYDROPHOBICITY OF CULTURED MAMMALIAN CELLS AND SOME EFFECTS OF BACTERIAL PHOSPHOLIPASES C
Author(s) -
MALMQVIST TORSTEN,
THELESTAM MONICA,
MöLLBY ROLAND
Publication year - 1984
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1984.tb02806.x
Subject(s) - chinese hamster ovary cell , cell , palmitic acid , biochemistry , cell membrane , bacterial cell structure , chemistry , membrane , cell culture , bacteria , enzyme , biology , chromatography , fatty acid , receptor , genetics
Eucaryotic cell surface hydrophobicity was measured as a partition of palmitic acid between the cell surface and the surrounding buffer. The method was found to be independent of cell mass or amount of palmitic acid within a large interval. An estimation of cell stability could also be obtained. The effects of Ca and Mg ions on cell hydrophobicity and stability of mouse myeloma (SP2/O) cells and of Chinese hamster ovary (CHO) cells were determined. This system permits measurement of changes in cell hydrophobicity caused by various additives, e.g. ions, purified bacterial products, antibiotics or cytostatics. Studies were made on these eucaryotic cells treated with purified bacterial phospholipases C from S. aureus and C. perfringens. These enzymes were found to increase the eucaryotic cell membrane hydrophobicity. This finding might indicate that bacterial phospholipases C facilitate bacterial colonization at the start of an infection.