Premium
STAPHYLOCOCCUS AUREUS PEPTIDOGLYCAN STUDIED BY ELECTRON MICROSCOPY OF IMMUNOPEROXIDASE‐TREATED SECTIONS OF CELL WALLS
Author(s) -
SOLSVIK EIMUND,
GROV ARNE,
ENDRESEN CURT
Publication year - 1984
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1984.tb02787.x
Subject(s) - peptidoglycan , immunoperoxidase , teichoic acid , cell wall , staphylococcus aureus , electron microscope , microbiology and biotechnology , staining , negative stain , bacterial cell structure , cell membrane , cell , homogeneous , chemistry , bacteria , biology , antibody , biochemistry , immunology , monoclonal antibody , optics , genetics , physics , thermodynamics
The immunoperoxidase technique in combination with electron microscopy was used for demonstration of antigenic determinants of Staphylococcus aureus peptidoglycan. Isolated antibodies against four synthetic peptides with amino‐acid sequences similar to those found in native staphylococcal peptidoglycans were used. A homogeneous staining of the cell wall from the outer surface to the cell membrane was demonstrated.