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C‐POLYSACCHARIDE IN A PNEUMOCOCCAL VACCINE
Author(s) -
SØRENSEN UFFE B. SKOV,
HENRICHSEN JØGEN
Publication year - 1984
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1984.tb00099.x
Subject(s) - polysaccharide , antiserum , antigen , chemistry , pneumococcal vaccine , cell wall , pneumococcal polysaccharide vaccine , streptococcus pneumoniae , microbiology and biotechnology , biochemistry , biology , pneumococcal disease , immunology , antibiotics
The 14‐valent pneumococcal vaccine, Pneumovax?, was found to contain approximately 25o ug per ml of the cell‐wall antigen, C‐polysaccharide (C‐Ps), in addition to 100 ug per ml of each of the 14 capsular polysaccharides. Rabbit anti‐C‐Ps antiserum recognized four components of this C‐Ps with different antigenic determinants, corresponding to four fragments with different molecular sizes. By lectin affinity chromatography it was demonstrated that some of the C‐Ps in the vaccine contained cell‐wall residues. The methods used in this study can also be used for the characterization of other polysaccharide preparations.