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IN VITRO INVESTIGATIONS OF HUMAN IMMUNOGLOBULIN PREPARATIONS: Fc‐RECEPTOR REACTIVITY AND COMPLEMENT CONSUMPTION
Author(s) -
RASMUSSEN J. MLLER,
BRANDSLUND I.,
SVEHAG S.E.
Publication year - 1984
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1984.tb00097.x
Subject(s) - in vitro , chemistry , antibody , complement (music) , complement system , receptor , biochemistry , complement component 5 , microbiology and biotechnology , immunology , biology , complementation , gene , phenotype
The complement consuming activity and monocyte Fc‐receptor (FcR) binding of some commercial IgG‐preparations for intraveneous (i.v.) and intramuscular (i.m.) use was investigated. A pepsin‐treated IgG‐preparation for i.v. use exhibited no detectable complement consuming activity and impaired FcR binding, whereas an unmodified IgG preparation had no detectable complement consuming activity, but retained full FcR binding. A preparation prepared by PEG‐precipitation and ion‐exchange chromatogra‐phy exhibited low complement consuming activity as well as good FcR binding. Three preparations for i.m. use showed moderate to strong complement consuming activity, whereas only one of these preparations retained full FcR binding.