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ROLE OF THE POLYSACCHARIDE AND OF THE LIPOPOLYSACCHARIDE IN THE IMMUNOPROTECTIVE CAPACITY OF KLEBSIELLA PNEUMONIAE RIBOSOMES
Author(s) -
ROBERT DANIEL,
NORMIER GERARD,
LAFONT SERGYL,
D'HINTERLAND LUCIEN DUSSOURD,
FONTANGES ROBERT,
WIGZELL HANS
Publication year - 1984
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1984.tb00090.x
Subject(s) - ribosome , rna , ribosomal rna , klebsiella pneumoniae , polysaccharide , lipopolysaccharide , microbiology and biotechnology , escherichia coli , biology , biochemistry , chemistry , gene , immunology
Ribosomes, ribosomal RNA (r‐RNA), capsular polysaccharide (PS‐K) and lipopolysaccharide (LPS) were isolated and purified from Klebsiella pneumoniae, type I. The protective capacity of these different fractions was investigated in function of their analytical composition. The results show that ribosomes, and in particular, ribosomal RNA have the greatest protective activity at the lowest concentrations. The role of PS‐K in the specific immune response to ribosomes was also investigated. Even at very high levels PS‐K failed to afford any significant protection. LPS gave no protection. A stable r‐RNA/PS‐K complex was isolated by means of affinity chromatography. This complex was uncontaminated by LPS and its existence indicated strong bonding between surface polysaccharide antigens and r‐RNA. The r‐RNA/PS‐K complex was not antigenic in itself but conferred good protection when combined with an adjuvant.