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CHLOROQUINE INHIBITS ACCESSORY CELL PRESENTATION OF SOLUBLE NATURAL AND SYNTHETIC PROTEIN ANTIGENS
Author(s) -
BUUS SOREN,
WERDELIN OLE
Publication year - 1984
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1984.tb00089.x
Subject(s) - chloroquine , antigen , antigen presentation , in vitro , proteolysis , antigen presenting cell , biology , antigen processing , immunology , microbiology and biotechnology , chemistry , pharmacology , cytotoxic t cell , biochemistry , malaria , enzyme
We have studied the in vitro effect of the lysosomotrophic agent, chloroquine, on the presentation of soluble protein antigens by guinea pig accessory cells. Chloroquine inhibited the capacity of antigen‐pulsed accessory cells to stimulate proliferation in appropriately primed T cells. The effect was time‐ and dose‐dependent. A brief treatment solely of the accessory cells with the drug compromised their ability to stimulate primed T cells in a subsequent culture provided the accessory cells were treated with chloroquine before their exposure to the antigen. These results suggest that chloroquine acts on an early event in the antigen handling by accessory cells. Chloroquine is a well known inhibitor of lysosomal proteolysis, and it is likely that its effect on antigen presentation is caused by an inhibition of antigen degradation.