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INHIBITION OF COMPLEMENT‐MEDIATED HEMOLYSIS IN GEL BY RHEUMATOID FACTORS
Author(s) -
TRUEDSSON LENNART,
SJÖHOLM ANDERS G.
Publication year - 1984
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1984.tb00044.x
Subject(s) - hemolysis , complement (music) , complement system , medicine , immunology , chemistry , antibody , biochemistry , gene , phenotype , complementation
When subjected to a hemolysis in gel (HIG) assay for the detection of complement deficiency, 9 of 37 sera from patients with classical rheumatoid arthritis produced impaired lysis of sensitized sheep erythrocytes. All sera were normal in a test for the alternative pathway and no major abnormalities were found within the complement system. Using a two‐step HIG technique, with guinea‐pig serum as the complement source, all sera were shown to inhibit lysis of sheep erythrocytes sensitized with rabbit IgG. Lysis of IgM‐coated erythrocytes was not inhibited. The agglutination titers in a Waaler‐Rose test, and the areas of inhibition in the two‐step HIG assay with IgG‐sensitized erythrocytes, were correlated (r = 0.80, p < 0.001). Absorption of serum with rabbit IgG coupled to Sepharose 4B, reduced the capacity to inhibit imḿune hemolysis. The eluate from IgG‐Sepharose contained rheumatoid factors and also inhibited immune hemolysis. The findings suggested that rheumatoid factors in serum were responsible for inhibition in the HIG assays used.