Premium
CORRELATION OF IMMUNOPHENOTYPE TO MORPHOLOGY IN UNFAVOURABLE NON‐HODGKIN LYMPHOMA
Author(s) -
Christensson Birger,
Lindemalm Christina,
Johansson Bo,
Mellstedt HåKan,
öST åKE,
Biberfeld Peter
Publication year - 1983
Publication title -
acta pathologica microbiologica scandinavica series a :pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0164
DOI - 10.1111/j.1699-0463.1983.tb02775.x
Subject(s) - immunophenotyping , lymphoma , pathology , histology , biology , nodular sclerosis , non hodgkin's lymphoma , antigen , medicine , immunology , hodgkin lymphoma
The relationship between immunological markers and histology according to the Kiel classification was studied in 40 adult non‐Hodgkin lymphoma patients of Rappaport unfavourable histology. The membrane‐associated and cytoplasmic Ig as well as receptors for sheep erythrocytes, Fcγ and C3d receptors were analyzed on cryostate sections and in suspension. In some cases, a more precise immunophenotype was achieved by the use of monoclonal antibodies detecting different T and B cell antigens. Eighty‐eight per cent of the lymphomas had B‐cell, five per cent T cell and 7 per cent non‐B/ non‐T cell phenotypes. All CBCC and CC lymphomas expressed monotypic Ig, but only 66 per cent of the CB lymphomas. Thus, morphology alone did not consistently predict immunophenotype in large‐cell lymphomas. A simultaneous expression of multiple heavy chains and cytoplasmic Ig was found in some lymphomas, suggesting an intratumoral differentiation. The nodular or irregular tissue distribution patterns for Ig and C3d receptors found in histologically diffuse follicle derived lymphomas also suggest intratumoral variations in the marker expression, probably related to differentiation. The results suggest that lymphoma immunophenotype is important in obtaining a definite diagnosis in large‐cell lymphomas and that it may lead to a better understanding of the differentiation of lymphomas of low‐grade malignancy.