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THE DETACHMENT, ATTACHMENT AND GROWTH CAPACITY OF HUMAN GLIOMA AND GLIA CELLS IN CULTURE
Author(s) -
Benediktsson Gudmundur,
Carlsson JöRgen,
Nilsson Sverker,
Jakobsson Per Åke,
Pontén Jan
Publication year - 1983
Publication title -
acta pathologica microbiologica scandinavica series a :pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0164
DOI - 10.1111/j.1699-0463.1983.tb02759.x
Subject(s) - glioma , clone (java method) , cloning (programming) , cell culture , ploidy , biology , cell , agarose , cell growth , microbiology and biotechnology , function (biology) , immunology , cancer research , genetics , dna , gene , computer science , programming language
The detachment, attachment and growth were studied in malignant glioma and normal glia cells in culture, using the palladium‐agarose cloning method. The number of cells in each individual clone was repeatedly counted throughout 10 days. All four glioma cell‐lines studied detached and attached themselves at higher rates than the normal, diploid, glia cells. This dynamic behaviour seemed to be a general property of cultured, malignant, glioma cells. All the cell‐lines contained clones with different properties. Some clones were growing, others were nearly constant in cell number and yet others decreased in cell number as a function of time. The differences between these three types of clones were surprisingly sharp.