Premium
SPECIFICITY OF CELLULAR INTERACTION IN STREPTOCOCCUS MITIS ATCC 903. INHIBITION OF AGGREGATION BY CARBOHYDRATES
Author(s) -
ABAAS SALEEM,
HOLME TORD
Publication year - 1983
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1983.tb00070.x
Subject(s) - chemistry , hexosamines , melibiose , biochemistry , galactosamine , streptococcus mitis , glucosamine , bacteria , streptococcus , biology , maltose , enzyme , genetics
To demonstrate the specificity of cellular interaction in the spontaneous aggregation of Streptococcus mitis ATCC 903 the inhibitory effect of various sugars was studied. More than 90% inhibition was recorded in 0.1 M concentrations of D‐glucosamine, D‐galactosamine and D‐mannosamine. A reduction of the inhibition by more than 50% was observed when the N‐acetylated derivatives of the hexosamines were tested. Polymers containing hexosamines such as hyaluronic acid, heparin and fetuin were potent inhibitors, in contrast to dextrans of different molecular weights. Inhibition was less than 20% in lactose, melibiose, cellobiose, methyl β‐D‐galactopyranoside and a number of other sugars tested in 0.1 M concentration. The bacteria retained their ability to aggregate after removal of the inhibitory sugars by washing in phosphate buffer. These findings support the hypothesis that the specific nature of the spontaneous aggregation of S.mitis ATCC 903 depends on a lectin‐ligand type interaction.