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ANTIBODY RESPONSE IN RABBITS TO GONOCOCCAL LIPOPOLYSACCHARIDE‐BOVINE SERUM ALBUMIN CONJUGATES
Author(s) -
RØDAHL EYVIND,
MÆLAND JOHAN A.
Publication year - 1983
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1983.tb00048.x
Subject(s) - bovine serum albumin , lipopolysaccharide , antibody , conjugate , serum albumin , immunology , albumin , chemistry , antibody response , microbiology and biotechnology , medicine , biology , biochemistry , mathematical analysis , mathematics
The elicitation of antibodies to gonococcal lipopolysaccharide (LPS) was studied. Rabbits were immunized with whole cells of gonococci, purified LPS, or LPS linked to bovine serum albumin with glutaraldehyde. Purified LPS was not immunogenic. Anti‐LPS antibodies were produced by rabbits receiving the LPS‐BSA conjugate. These animals showed an earlier IgG anti‐LPS antibody response than animals receiving whole bacterial cells. Antiserum to the LPS‐BSA conjugate gave rise to a single precipitation line against ultrasonically disrupted gonococci, and agglutinated heat‐treated cells of the bacteria.