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ADHERENCE AND TOXICITY OF YERSINIA ENTEROCOLITICA 0:3 AND 0:9 CONTAINING VIRULENCE‐ASSOCIATED PLASMIDS FOR VARIOUS CULTURED CELLS
Author(s) -
VESIKARI TIMO,
SUNDQVIST CARIN,
MÄKI MARKKU
Publication year - 1983
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1983.tb00020.x
Subject(s) - yersinia enterocolitica , virulence , microbiology and biotechnology , plasmid , bacteria , enterobacteriaceae , phagocytosis , biology , in vitro , yersinia , toxicity , escherichia coli , chemistry , gene , biochemistry , genetics , organic chemistry
Plasmid (47 and 44 Mdal, respectively) containing strains of Yersinia enterocolitica 0:3 and 0:9 adhered to and were toxic for HEp‐2 human epithelial and Y‐1 adrenal cells in vitro. At 37 °C, but not at room temperature, the adhesion of the bacteria lead to rounding and partial detachment of the cultured cells. UV‐inactivated plasmid‐positive Y. enterocolitica were neither adherent nor toxic for the cells but were readily endocytosed by HEp‐2 cells. The adherence of plasmid‐positive Y. enterocolitica 0:3 and 0:9 on epithelial cells may be pathogenetically important as an initial step for intestinal colonization, and possibly in Y. enterocolitica ‐induced diarrhoea. Plasmid‐positive Y. enterocolitica also adhered to the surface of cultured human macrophages and were apparently not phagocytosed as effectively as the plasmid‐negative derivatives of the same bacteria. Thus resistance to phagocytosis may form an additional plasmid‐dependent virulence property of Y. enterocolitica 0:3 and 0:9.